Oxford Expression Technologies - Transfection Reagents
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Information on the Oxford Expression Technologies Transfection Reagents
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baculoFECTIN II™and flashFECTIN™ are novel nanoparticle based transfection reagents that have been specifically developed and optimised for the transfection of insect cells. It is ideal for baculovirus work and is effective with all insect cell lines including Sf9 and Sf21.
 
• RELIABLE - Tried and tested and is regularly used in-house in the Oxford Expression Technologies laboratories
• EFFICIENT - Tests have shown reproducibly higher levels of transfection in insect cells to facilitate high level protein expression
• SIMPLE - Quick and easy protocol: from co-transfection to high titre seed stock
• CELL FRIENDLY - Completely non-toxic so cell health and viability are maintained
Specific Product/Application Areas
Go to flashBAC Product Information flashBAC Baculovirus Protein Expression
Go to baculoQUANT Product Information baculoQUANT Virus Titration
Go to titrePLUS Product Information titrePLUS Virus Titration and Protein Expression
Go to pOET Transfer Plasmids Product Information pOET Transfer Plasmids
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Go to NEW flashBAC™ Selection Box NEW flashBAC™ Selection Box
Go to NEW 96 Reaction flashBAC™ Kits NEW 96 Reaction flashBAC™ Kits
Literature and Technical Requests
Go to flashBAC Technical Literature Download Technical Literature
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Product Technical Overview
baculoFECTIN II

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Excellent Transfection Efficiency – Easily Produce High Titre Virus Stocks

baculoFECTIN II is a transfection reagent ideally suited for use with insect cell lines.

Although optimised for insect cell lines such as Sf9, Sf21 and T.ni, the reagent will also work with other cell lines and primary cells too, including various mammalian cell lines.  baculoFECTIN II consists of two components, a positively charged polymer with DNA-binding capacity that is embedded into the second component, a novel porous nanoparticle. The complete nanoparticle complex protects the bound DNA from degradation by nucleases. Only the DNA nanoparticle complex with its unique size can be preferentially taken up by the cells. Within the cells, the released DNA-nanoparticle complexes are protected against DNA degradation.
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flashFECTIN

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flashFECTIN is a non-lipid based formulation, optimised for transfection of insect cell lines. Non-toxic to cells. With a highly streamlined, rapid protocol co-transfection reactions with the flashBAC system have never been faster. Manufactured to the highest standards to ensure lot-to-lot consistency.
 
Features:
• Rapid, easy to use protocol
• Efficient transfection using flashBAC™ baculovirus expression system
• Non-toxic to cells
• Optimised for insect cell lines Sf9, Sf21 and Tni
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