Oxford Genetics - Products and Applications
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Oxford Genetics range of high quality  DNA Plasmid Vectors
Go to Oxford Genetics website
Assemble the plasmid you need from the Oxford Genetics catalogue of over 1,500 plasmids.
 
All of the plasmids from Oxford Genetics are based on the same backbone, allowing the construction of any plasmid using the thousands of pre-made DNA components.
 
The Oxford Genetics concept is simple: One plasmid, a thousand possibilities.
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Pricing and Availability: Please contact us for pricing and availability on any of the Oxford Genetic's range of products.
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For detailed information go to the Oxford Genetics website:
Go to Oxford Genetics website
www.oxfordgenetics.com
About SnapFast™ Plasmids
Genetic engineering is used in thousands of laboratories around the world. Given its scientific and commercial importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. The aim of Oxford Genetics was to engineer a DNA plasmid system that could accommodate most of the functional DNA inserts that a researcher might require within a single plasmid. By optimising their starting vector, every component of the system can be removed and exchanged for hundreds of other DNA sections which have been pre-designed and tested by Oxford Genetics. This is the concept of the SnapFast™ system.
 
Cleaner DNA - All of the constructs from Oxford Genetics have been pre-screened for poor codon usage and conflicting restriction sites. Where possible, all rare codons and restriction sites have been removed to enable efficient expression, and ensure that conflicting restriction sites do not limit the cloning of other SnapFast™ DNA inserts.
 
Incredible Versatility Oxford Genetics has one of the largest collections of plasmids available globally, providing multiple expression and cloning options for almost every insert we provide. Their product range includes:
  • The largest peptide tag range in the world
  • More than 9 reporter genes in 5 configurations
  • More than 50 signal peptides
  • Over 40 promoters for mammalian, bacteria and yeast expression
  • 10 antibiotic and metabolic selection options
  • 6 origins of replication and a range of other DNA sections
 
Synthetic Biology is an emerging field that is set to change the way scientists work with DNA. Plasmids from Oxford Genetics are uniquely designed to allow the creation of complex DNA expression vectors with minimal cloning steps. This feature makes them the ideal tool set for synthetic biology applications and enables Oxford Genetics to offer versatile cloning systems that deliver results, regardless of a projects complexity.
 
Always Flexible - Whilst most of the DNA sections within their plasmids are flanked by restriction sites of 6 base pairs (bp) in length, they have also strategically placed 8 bp restriction sites at key positions throughout the plasmid to maximise the versatility of the system. The low frequency of each 8 bp cutting site in random DNA makes it unlikely that any gene inserted will contain such a site. By exploiting this principle DNA plasmids from Oxford Genetics have limitless potential and unparalleled flexibility.
 
Primary Benefits of the SnapFast™ System:
  • Universal cloning flexibility.
  • Unrivalled access to DNA variants and sequences.
  • Easy, efficient, and simple engineering strategies.
  • High success rates due to compatible inserts.
  • No insert size constraints.
  • Compatible with many pre-existing cloning vectors (T0P0, pGL3, C1-EGFP etc) and a range of shuttle vectors to facilitate gene transfer.
  • Easy cloning from the SnapFast™ vectors into a range of alternative systems, including viral vectors.
  • Built in regulatory sequences and capabilities (e.g. gene concatamerisation, integrated insulators, T7 and bacterial terminator).
Example of a SnapFast™ Plasmid
Vector: pSF-CMV-PGK-daGFP

Product Code: OG390

Quantity Provided: 5µg

Size (bp): 5452

Bacterial Antibiotic Selection: Kanamycin

Origin and Compatibility: pUC high copy, derived from pBR322 

Copy Number: 500-700 copies per cell

Promoter:  Cytomegalovirus (CMV) immediate early promoter and the mouse phosphoglycerate kinase promoter (PGK)

Purpose:  This plasmid contains two promoters that terminate transcription at the same poly-adenylation signal, allowing the expression of two genes from one expression cassette, where the second gene is the  daGFP fluorescent reporter gene. The second promoter (PGK) is approximately 10-fold weaker than the upstream CMV promoter in most commonly used cell lines.

daGFP: This vector encodes a green fluorescent protein called daGFP. This protein can be used in the same way as normal GFP using argon laser based or UV based excitation apparatus to allow the detection of fluorescence. The protein has a peak excitation of 510nm and a peak emission of 521nm. The gene encoding the protein is sold free of intellectual property restrictions.  

Quality Validation: This vector has been demonstrated to express reporter genes to high levels under the CMV promoter. The start codon of these reporters was positioned within the NcoI site and the stop codon was positioned within the XbaI site. This plasmid also demonstrates intermediate levels of expression of daGFP from the PGK promoter. Expression has been validated in 293 cells.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through Oxford Genetics website.

Intellectual Property Status: According to the Oxford Genetics IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.
Genetic Modifications to standard parts:
  • CMV promoter – an NcoI site has been ablated in the CMV promoter. This change does not reduce expression.
  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFast system and to retain a high level of antibiotic resistance. It has been validated in E.coli.
  • daGFP – The daGFP reporter gene has been modified to remove PciI and BseRI restriction sites.
 
Restriction site notes:
  • Bgl2, KpnI, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, start codon, origin, and KanR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.
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